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Biotechnology Information primer blast online tool
Primer Blast Online Tool, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer-blast+online+tool/blast+primer/pm42278052-144-13-21
Average 86 stars, based on 1 article reviews
primer blast online tool - by Bioz Stars, 2026-10
86/100 stars

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Article Title: RABL6A Promotes Pancreatic Neuroendocrine Tumor Angiogenesis and Progression In Vivo
Article Snippet: The National Center of Biotechnology Information (NCBI) Primer-BLAST online tool was used to design primers specific for the mouse genes of interest. (a) Hprt (housekeeping gene used for normalization): forward- 5′ GCCCCAAAATGGTTAAGGTTG 3′ , reverse- 5′ TGGCCTGTATCCAACACTTC 3′ ; (b) c-Myc : forward- 5′ CCTGTACCTCGTCCGATTCC 3′ , reverse- 5′ TTCTTGCTCTTCTTCAGAGTCG 3′ ; (c) Vegfa : forward- 5′ AGCAGATGTGAATGCAGACCA 3′ , reverse- 5′ GACCCAAAGTGCTCCTCGAA 3′ ; and (d) Arf - forward- 5′ CGGAATCCTGGACCAGGTG 3′ , reverse- 5′ ACCAGCGTGTCCAGGAAGC 3′ .

Article Title: Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood
Article Snippet: Primer pair specificity for all designed primers was checked using the National Center for Biotechnology Information (NCBI) Primer-BLAST online tool ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ).

Article Title: Transcriptional network analysis of peripheral blood leukocyte subsets in multiple sclerosis identifies a pathogenic role for a cytotoxicity-associated gene network in myeloid cells.
Article Snippet: Primers specific for target genes’ transcript, off-target transcript and house-keeping control were designed according to standard PCR primer design parameters using the PRIMER-BLAST online tool available on the National Center for Biotechnology Information (NCBI) website (www.ncbi.nlm.nih.gov), with preference for at least one primer to span an exon–exon junction, producing a single amplicon product size in the range of 50–200 bp and melting temperature of 60°C.

Article Title: Transcriptional heterogeneity of fibroblasts is a hallmark of the aging heart.
Article Snippet: Primer sequences were designed using the National Center for Biotechnology Information Primer-BLAST online tool and were purchased from MilliporeSigma (a full list can be found in Supplemental Table 9).

Article Title: Chalcone Synthase-Encoding AeCHS is Involved in Normal Petal Coloration in Actinidia eriantha
Article Snippet: Primers used for quantitative real-time polymerase chain reaction (qRT-PCR) were designed using Primer-Blast online tool in the National Center for Biotechnology Information (NCBI, https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ).

Article Title: One-Step Triplex High-Resolution Melting Analysis for Rapid Identification and Simultaneous Subtyping of Frequently Isolated Salmonella Serovars
Article Snippet: The Primer-BLAST online tool from the National Center for Biotechnology Information (NCBI) ( http://www.ncbi.nlm.nih.gov ) was used for specific HRM primer design.

Article Title: Role of SiPHR1 in the Response to Low Phosphate in Foxtail Millet via Comparative Transcriptomic and Co-Expression Network Analyses
Article Snippet: The primer-BLAST online National Center for Biotechnology Information (NCBI) tool was used to design primers specific for each DEG ( ).

Article Title: Brief antibiotic use drives human gut bacteria towards low-cost resistance.
Article Snippet: Understanding the relationship between antibiotic use and the evolution of antimicrobial resistance is vital for effective antibiotic stewardship.. Yet, animal models and in vitro experiments poorly replicate real-world conditions.. To explain how resistance evolves in vivo, we exposed 60 human participants to ciprofloxacin and used longitudinal stool samples and a new computational method to assemble the genomes of 5,665 populations of commensal bacterial species within participants.

Polymerase Chain Reaction:

Article Title: RABL6A Promotes Pancreatic Neuroendocrine Tumor Angiogenesis and Progression In Vivo
Article Snippet: The National Center of Biotechnology Information (NCBI) Primer-BLAST online tool was used to design primers specific for the mouse genes of interest. (a) Hprt (housekeeping gene used for normalization): forward- 5′ GCCCCAAAATGGTTAAGGTTG 3′ , reverse- 5′ TGGCCTGTATCCAACACTTC 3′ ; (b) c-Myc : forward- 5′ CCTGTACCTCGTCCGATTCC 3′ , reverse- 5′ TTCTTGCTCTTCTTCAGAGTCG 3′ ; (c) Vegfa : forward- 5′ AGCAGATGTGAATGCAGACCA 3′ , reverse- 5′ GACCCAAAGTGCTCCTCGAA 3′ ; and (d) Arf - forward- 5′ CGGAATCCTGGACCAGGTG 3′ , reverse- 5′ ACCAGCGTGTCCAGGAAGC 3′ .

Article Title: Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood
Article Snippet: Primer pair specificity for all designed primers was checked using the National Center for Biotechnology Information (NCBI) Primer-BLAST online tool ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ).

Article Title: Transcriptional network analysis of peripheral blood leukocyte subsets in multiple sclerosis identifies a pathogenic role for a cytotoxicity-associated gene network in myeloid cells.
Article Snippet: Primers specific for target genes’ transcript, off-target transcript and house-keeping control were designed according to standard PCR primer design parameters using the PRIMER-BLAST online tool available on the National Center for Biotechnology Information (NCBI) website (www.ncbi.nlm.nih.gov), with preference for at least one primer to span an exon–exon junction, producing a single amplicon product size in the range of 50–200 bp and melting temperature of 60°C.

Article Title: Transcriptional heterogeneity of fibroblasts is a hallmark of the aging heart.
Article Snippet: Primer sequences were designed using the National Center for Biotechnology Information Primer-BLAST online tool and were purchased from MilliporeSigma (a full list can be found in Supplemental Table 9).

Article Title: Chalcone Synthase-Encoding AeCHS is Involved in Normal Petal Coloration in Actinidia eriantha
Article Snippet: Primers used for quantitative real-time polymerase chain reaction (qRT-PCR) were designed using Primer-Blast online tool in the National Center for Biotechnology Information (NCBI, https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ).

Article Title: One-Step Triplex High-Resolution Melting Analysis for Rapid Identification and Simultaneous Subtyping of Frequently Isolated Salmonella Serovars
Article Snippet: The Primer-BLAST online tool from the National Center for Biotechnology Information (NCBI) ( http://www.ncbi.nlm.nih.gov ) was used for specific HRM primer design.

Article Title: Role of SiPHR1 in the Response to Low Phosphate in Foxtail Millet via Comparative Transcriptomic and Co-Expression Network Analyses
Article Snippet: The primer-BLAST online National Center for Biotechnology Information (NCBI) tool was used to design primers specific for each DEG ( ).

Article Title: Brief antibiotic use drives human gut bacteria towards low-cost resistance.
Article Snippet: Understanding the relationship between antibiotic use and the evolution of antimicrobial resistance is vital for effective antibiotic stewardship.. Yet, animal models and in vitro experiments poorly replicate real-world conditions.. To explain how resistance evolves in vivo, we exposed 60 human participants to ciprofloxacin and used longitudinal stool samples and a new computational method to assemble the genomes of 5,665 populations of commensal bacterial species within participants.

Amplification:

Article Title: RABL6A Promotes Pancreatic Neuroendocrine Tumor Angiogenesis and Progression In Vivo
Article Snippet: The National Center of Biotechnology Information (NCBI) Primer-BLAST online tool was used to design primers specific for the mouse genes of interest. (a) Hprt (housekeeping gene used for normalization): forward- 5′ GCCCCAAAATGGTTAAGGTTG 3′ , reverse- 5′ TGGCCTGTATCCAACACTTC 3′ ; (b) c-Myc : forward- 5′ CCTGTACCTCGTCCGATTCC 3′ , reverse- 5′ TTCTTGCTCTTCTTCAGAGTCG 3′ ; (c) Vegfa : forward- 5′ AGCAGATGTGAATGCAGACCA 3′ , reverse- 5′ GACCCAAAGTGCTCCTCGAA 3′ ; and (d) Arf - forward- 5′ CGGAATCCTGGACCAGGTG 3′ , reverse- 5′ ACCAGCGTGTCCAGGAAGC 3′ .

Article Title: Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood
Article Snippet: Primer pair specificity for all designed primers was checked using the National Center for Biotechnology Information (NCBI) Primer-BLAST online tool ( https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ).

Article Title: Transcriptional network analysis of peripheral blood leukocyte subsets in multiple sclerosis identifies a pathogenic role for a cytotoxicity-associated gene network in myeloid cells.
Article Snippet: Primers specific for target genes’ transcript, off-target transcript and house-keeping control were designed according to standard PCR primer design parameters using the PRIMER-BLAST online tool available on the National Center for Biotechnology Information (NCBI) website (www.ncbi.nlm.nih.gov), with preference for at least one primer to span an exon–exon junction, producing a single amplicon product size in the range of 50–200 bp and melting temperature of 60°C.

Article Title: Transcriptional heterogeneity of fibroblasts is a hallmark of the aging heart.
Article Snippet: Primer sequences were designed using the National Center for Biotechnology Information Primer-BLAST online tool and were purchased from MilliporeSigma (a full list can be found in Supplemental Table 9).

Article Title: Chalcone Synthase-Encoding AeCHS is Involved in Normal Petal Coloration in Actinidia eriantha
Article Snippet: Primers used for quantitative real-time polymerase chain reaction (qRT-PCR) were designed using Primer-Blast online tool in the National Center for Biotechnology Information (NCBI, https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ).

Article Title: One-Step Triplex High-Resolution Melting Analysis for Rapid Identification and Simultaneous Subtyping of Frequently Isolated Salmonella Serovars
Article Snippet: The Primer-BLAST online tool from the National Center for Biotechnology Information (NCBI) ( http://www.ncbi.nlm.nih.gov ) was used for specific HRM primer design.

Article Title: Role of SiPHR1 in the Response to Low Phosphate in Foxtail Millet via Comparative Transcriptomic and Co-Expression Network Analyses
Article Snippet: The primer-BLAST online National Center for Biotechnology Information (NCBI) tool was used to design primers specific for each DEG ( ).

Article Title: Brief antibiotic use drives human gut bacteria towards low-cost resistance.
Article Snippet: Understanding the relationship between antibiotic use and the evolution of antimicrobial resistance is vital for effective antibiotic stewardship.. Yet, animal models and in vitro experiments poorly replicate real-world conditions.. To explain how resistance evolves in vivo, we exposed 60 human participants to ciprofloxacin and used longitudinal stool samples and a new computational method to assemble the genomes of 5,665 populations of commensal bacterial species within participants.



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